An improved high-performance liquid chromatographic method for quantifying p-aminobenzoic acid and some of its metabolites
Brown, N.D.; Michalski, E.J.
Journal of Chromatography 121(1): 76-78
1976
ISSN/ISBN: 0021-9673 PMID: 932136 Document Number: 100753
The high-performance liquid chromatographic separation of p-aminobenzoic acid and 3 of its metabolites, N-acetyl-p-aminobenzoic acid, p-aminohippuric acid and N-acetyl-p-aminobenzoic acid, p-aminohippuric acid and N-acetyl-p-aminohippuric acid on an anionic silica gel micro-Partisil column, is described. Human serum and urine specimens can be analyzed directly without pretreatment, with all 4 compounds eluting within 12 min. The increased simplicity of the method, the lower operating pressures and less sophisticated instrumentation provide a routine test procedure for the clinical laboratory.