High performance liquid chromatographic method fo pyrantel tartrate in swine feeds and supplements
Goras, J.T.
Journal - Association of Official Analytical Chemists 64(6): 1291-1296
1981
ISSN/ISBN: 0004-5756 PMID: 7309651 Document Number: 174126
A new method for the determination of pyrantel tartrate in swine feed and supplements was developed because the current official AOAC [Association of Official Analytical Chemists] method was not applicable to feeds co-medicated with tylosin. The new method involved leaching of drug from feed with methanolic NaCl solution, removal of interfering substances by ion pair liquid-liquid extraction and high performance liquid chromatography, and quantitation of pyrantel tartrate by monitoring UV absorption of the effluent stream at 313 nm. The method of standard addition was used to compensate for the effect of the feed matrix on drug recovery. No interference was encountered from tylosin, carbadox, lincomycin, non-drug components of feeds and supplements, or potential degradation products of pyrantel tartrate, i.e., cis pyrantel tartrate and (E)-N-(3-methylaminopropyl)-2-thiopheneacrylamide. Results for the assay of 3 lots each of feeds and supplements containing 0.0106 and 0.106% pyrantel tartrate, respectively, were within .+-. 4% of label claim. Coefficients of variation ranged from 1.6-1.8% for feeds and 1.9-3.9% for supplements.