Rapid method for the simultaneous analysis of hydrocortisone and clioquinol in topical preparations by high-performance liquid chromatography
Phoon, K.W.; Stubley, C.
Journal of Chromatography 246(2): 297-303
1982
ISSN/ISBN: 0021-9673 PMID: 6216261 Document Number: 190793
Reversed-phase high-performance liquid chromatographic methods for the analysis of ointments containing hydrocortisone and clioquinol were investigated. A successful method using a C18 column and methanol-0.05 M phosphoric acid (80:20) as eluting solvent was developed which allows both compounds to be determined simultaneously. The high-performance liquid chromatographic procedure is rapid and sensitive. The method was also applied to the analysis of ointments containing hydrocortisone combined with other halogenated hydroxyquinolines. [The use of the antiinflammatory drug hydrocortisone together with the antibacterial compound clioquinol (5-chloro-7-iodo-8-quinolinol) is a well-recognized regime for the treatment of skin disorders.].