A rapid method for the determination of cyclosporin a in blood by high performance liquid chromatography
Toulet, V.; Saux, M.C.; Rouquette, C.; Penouil, F.; Brachet-Liermain, A.
Annales de Biologie Clinique 44(5): 517-521
1986
ISSN/ISBN: 0003-3898 PMID: 3813137 Document Number: 276997
The purpose of this paper is to propose a method for the determination of cyclosporin in whole blood by means of HPLC with UV detection. After a one step extraction into ether, the organic layer is washed NaOH and evaporated to dryness. The residue is reconstituted in a mixture of acetonitrile and water (40/60) (V/V) and injected into the chromatograph. The C8 reversed phase column is warming at 75 degrees C temperature. The mobile phase consists of acetonitrile and water (60/40) (V/V) and the wavelength is set at 210 nm. The technique is rapid, sensitive, reproducible and well suited for therapeutic monitoring and for pharmacokinetic studies.