Method for simultaneous determination of creatine phosphate and adenine nucleotides in the intestinal smooth muscle of guinea-pig taenia caeci using high-performance liquid chromatography
Endo, I.; Utsugi, M.; Suzuki, T.; Karaki, H.
Nihon Heikatsukin Gakkai Zasshi 24(2): 127-130
1988
ISSN/ISBN: 0374-3527 PMID: 3244213 Document Number: 325330
A method for simultaneous determination of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP) and creatine phosphate (PCr) by high-performance liquid chromatography is described. This method was applied to the isolated intestinal smooth muscle tissue of guinea pig taenia caeci weighing approximately 30 mg. It was found that one g of the muscle tissue contained 3.55 .mu.mol PCr, 2.40 .mu.mol ATP and 0.477 .mu.mol ADP.