Cryopreservation of the human multipotent stem cell
Fabian, I.; Douer, D.; Wells, J.R.; Cline, M.J.
Experimental Hematology 10(1): 119-122
1982
ISSN/ISBN: 0301-472X PMID: 7060656 Document Number: 186464
Light density human bone marrow cells were cryopreserved, then thawed and tested for their ability to form myeloid (CFUGM), erythroid (BFUE) and mixed myeloid-erythroid colonies in vitro. Proliferation of mixed hematopoietic colonies was supported by conditioned media from a human T-lymphocyte cell line and erythropoietin. All mixed colonies contained neutrophil and erythroid precursors and approximately 25% had mononuclear phagocytes. Following cryopreservation the bone marrow retained its capacity to form mixed colonies without apparent loss. The recovery of CFUGM and BFUE was 76% and 72% respectively.