Culturing and cryopreservation of pre-cartilaginous stem cells from neonate rat
You, H.; Chen, A.; Cheng, H.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 19(3): 238-240
2005
ISSN/ISBN: 1002-1892 PMID: 15828484 Document Number: 593255
To establish an effective way to cryopreserve pre-cartilaginous stem cells (PSCs) of neonate rat. PSCs [fibroblast growth factor-3 (FGFR-3) positive cells] were isolated and purified by magnetic cell sorting method. PSCs were cultured and amplified to the third generation. PSCs were preserved in liquid nitrogen. The biological properties of cryopreserved PSCs were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), immunohistochemistry, and immunofluorescence. Immunohistochemical and immunofluorescent analysis showed widespread expression of FGFR-3 in cryopreserved PSCs. FGFR-3 could be detected by RT-PCR in cryopreserved PSCs. Cryopreserved PSCs kept high cell viability, and phenotypic and proliferation characteristics of PSCs in vivo. Cryopreservation of PSCs can supply adequate qualified cells for repairing the defects of epiphyseal growth plate by tissue engineering technique.