The mechanism of stem cell factor in bone marrow stem cell transplantation to heart
Liu, Y-gang.; Guo, J-xuan.; Zhang, P.; Li, Z-Ping.
Zhonghua Xin Xue Guan Bing Za Zhi 33(10): 927-931
2005
ISSN/ISBN: 0253-3758 PMID: 16266484 Document Number: 594917
Objective To study the mechanism of stem cell factor (SCF) in bone marrow stem cells heart transplantation (BMT) and the influence of bone marrow mobilization on the transplantation efficacy. Methods Rats with acute myocardial infarction (AMI) accepted BMT. The SCF expression in the bone marrow was measured by RT-PCR after the operation. Then bone marrow stem cells with different SCF levels for the transplantation were used and the cardiac function was compared by using echocardiography. The SCF protein expression in the heart, plasma and bone marrow was detected by ELISA. Results SCF expression level decreased significantly 1 week after AMI (P < 0.01), but it didn't decrease in those accepting BMT. Though the rats accepted BMT with bone marrow stem cells from different sources, the cardiac function showed no difference (P > 0.05). After BMT, the SCF protein level in the plasm decreased significantly (P < 0.05). Conclusions BMT may make mobilization through SCF. Bone marrow stem cells from rats with AMI and also those with myocardial infarction plus BMT therapy can also be used for the transplantation into heart, and have no influence on cardiac function improvement.