Specific effect of endonucleases from Brevibacterium ammoniagenes on DNA
Basnak'ian, A.G.; Botrin, I.I.; Debov, C.C.
Biokhimiia 46(7): 1307-1314
1981
ISSN/ISBN: 0320-9725 PMID: 6268197 Document Number: 180990
The previously described deoxyribonucleases from Brevibacterium ammoniagenes have been characterized. It was shown that they are endonucleases with molecular weights of 60 000 (I), 10 000 (II) and 20 000 (III). The rate of endonuclease I effect on native DNA exceeded that on the denatured DNA 2-fold. The mechanism of its action is of a single hit type. The enzyme hydrolyzes two chains of DNA simultaneously in two symmetrical sites and splits the bond 5'-P to form fragments with terminal 5'-OH and 3'-P. Endonuclease I was characterized as deoxyribonucleate-3'-oligonucleotide hydrolase (EC 3.1.4.6).