Restriction endonucleases from Bifidobacteria
Skrypina, N.A.; Kramarov, V.M.; Liannaia, A.M.; Smolianinov, V.V.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1988(5): 15-16
1988
ISSN/ISBN: 0208-0613 PMID: 2842668 Document Number: 323922
The sitespecific restriction endonucleases were found in four strains among the twelve strains of anaerobic bacteria of generum Bifidobacterium. Two of the restriction endonucleases studied, BadI from B. adolescentis LVA1 and BbfI from B. bifidum LVA3, are isoshizomers of XhoI and recognize the nucleotide sequence CTCGAG. The restriction endonucleases Bbf7411I from B. bifidum 7411 and Bla7920I from B. lactentis 7920 recognize and hydrolyze the nucleotide sequence TCCGGA having the specificity analogous to the one of restriction endonuclease CauB3I. Like CauB3I, these restriction endonucleases are unable to hydrolyze DNA if the adenine residues in the recognition site are methylated.