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Design of a nutrient medium for bacteria of the genus Haemophilus--producers of restriction endonucleases

Poliachenko, V.M.; Mel'nikova, V.A.; Gruber, I.M.; Smirnova, G.A.; Raskin, B.M.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 3-8

1989

ISSN/ISBN: 0372-9311
PMID: 2545058
Document Number: 346531
A culture medium for the cultivation of hemophilic bacteria, containing acidic casein hydrolysate, aminopeptide and fodder yeast extract, has been proposed. The growth-stimulating properties of this medium have been studied on 5 strains producing restrictases differing in their specificity. In growing these producer strains in a model ANKUM-2 fermenter with the supply of carbohydrate substrates (glucose, sucrose, glycerin) the yield of biomass, considered to be high for hemophilic bacteria (10-14 g of humid substance from 1 liter of the medium), has been achieved. As shown on H. influenzae Rc B-2297 used as an example, an increase in the yield of microbial biomass leads to a decrease in restrictase specific activity.

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