Diagnosis of experimental bovine cysticercosis using the ELISA test
Chroustová, E.
Veterinarni Medicina 26(12): 721-730
1981
ISSN/ISBN: 0375-8427 PMID: 6800104 Document Number: 178823
Seven calves of the Bohemian Spotted and Black-Pied Lowland breeds, aged two to three months and a half, were experimentally invaded per os with 5 000, 10 000, 25 000, 70 000, 95 000, 113 000 and 120 000 eggs of Taenia saginata. In the calf invaded with 70 000 eggs, antibodies were detected by the ELISA method already a week from invasion and in the remaining calves two to three weeks from invasion. The increase in the content of antibodies in the blood serum of the invaded animals was statistically significant from the fourth to the tenth week from invasion when the titre was 1 : 160 to 1 : 320 and when the measured extinction values ranged from 0.53 to 0.58. The antibody level remained significant and positive for seven months and a half to nine months. Clinical symptoms were observed in the calves invaded with 25 000 to 120 000 eggs for a period of 7 to 20 days from the 10th to 11th or 19th day after invasion. In the remaining calves the clinical symptoms were not significant. The post-mortal examination of four calves showed 2.1 to 3.9% of cysticerci from the applied eggs. The ELISA enzymatically immunological method, which was used for the first time in the Czech Socialist Republic in the case of cysticercosis, is suitable for detecting antibodies to the parasite Cysticercus bovis in the blood serum of calves experimentally invaded with the eggs of the tapeworm Taenia saginata.