Preparative liquid chromatography of 1:1 adducts derived from the reaction of malondialdehyde with amino acids
Pietrzyk, D.J.; Stodola, J.
Analytical Biochemistry 117(2): 245-249
1981
ISSN/ISBN: 0003-2697 PMID: 7325364 Document Number: 171377
Purification of 1:1 adducts (enaminals) formed from the reaction of methyl esters of amino acids with either malondialdehyde or methylmalondialdehyde was accomplished by preparative liquid chromatography on the stationary phase Amberlite XAD-4. The enaminal was easily and rapidly separated from the by-products and starting materials by a EtOH-H2O mobile phase and then easily and rapidly recovered from the EtOH-H2O at good yield and high purity. Depending on column diameter, up to 50 mg of enaminal could be purified in 1 chromatographic step. Adducts derived from L-Arg, L-His, L-Tyr and L-Cys were purified.