Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene
Gething, M.J.; Sambrook, J.
Nature 293(5834): 620-625
1981
ISSN/ISBN: 0028-0836 PMID: 6270568 Document Number: 169611
By replacing either the early or the late genes of SV40 with a cloned copy of the influenza virus hemagglutinin gene, recombinant viruses were constructed which, in infected cells, express large quantities of hemagglutinin. This glycoprotein, over 108 molecules of which are produced/cell, is identical in MW to authentic influenza virus hemagglutinin, accumulates at the cell [African green monkey kidney CV-1] surface and displays hemabsorbing activity.
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