Alloreactive cloned T cell lines. III. Accessory cell requirements for the growth of cloned cytolytic T lymphocytes
Lutz, C.T.; Glasebrook, A.L.; Fitch, F.W.
Journal of Immunology 126(4): 1404-1408
1981
ISSN/ISBN: 0022-1767 PMID: 6970775 Document Number: 174331
We examined the role of accessory cells that cooperated with the cloned cytolytic T cell line, L3. Accessory cells and T cell growth factors (TCGF) acted synergistically to stimulate cloned L3 CTL to grow and produce cytolytic activity. Accessory cells were non-T, radioresistant, plastic adherent M phi-like cells that interacted with cloned L3 CTL via soluble factors. Peritoneal M phi, at low density, also cooperated with L3 cells but at high density, inhibited L3 cells. Both accessory and inhibitory M phi acted upon L3 CTL without the intervention of other T lymphocytes or nonadherent cells. Other published reports indicate that M phi may promote cytolytic activity indirectly, amplifying TCGF production by helper T lymphocytes. This report indicates that M phi may also promote cytolytic activity directly, amplifying the response of CTL to TCGF.