Alloreactive cloned T cell lines. VI. Multiple lymphokine activities secreted by helper and cytolytic cloned T lymphocytes
Prystowsky, M.B.; Ely, J.M.; Beller, D.I.; Eisenberg, L.; Goldman, J.; Goldman, M.; Goldwasser, E.; Ihle, J.; Quintans, J.; Remold, H.; Vogel, S.N.; Fitch, F.W.
Journal of Immunology 129(6): 2337-2344
1982
ISSN/ISBN: 0022-1767 PMID: 6216281 Document Number: 188078
Culture supernatants generated by alloantigenic or lectin stimulation of a cloned helper T lymphocyte, designated L2, contain interleukin 2 (IL 2), granulocyte/macrophage colony-stimulating factor (CSF), B cell stimulating factor (BCSF), macrophage (Ia+)-recruiting factor, (Ia+)-inducing activity, .gamma.-interferon, Fc receptor-enhancing activity, macrophage migration inhibitory factor, macrophage activation factor (MAF), interleukin 3 (IL 3), and a factor responsible for prolonging the synthesis and secretion of the 4th and 2nd complement components by guinea pig peritoneal macrophages. Erythropoietin was not detected. A spontaneously arising variant of L2, designated L2V, produces much lower quantities of macrophage-stimulating activities, IL 2 and interferon. When compared to L2, L2V produces much higher levels of BCSF, equivalent amounts of IL 3, and slightly smaller amounts of CSF. Unlike L2V, a cytolytic clone designated L3 secretes lymphokines that primarily affect macrophage function. The time course of lymphokine production by L2 cells indicates that for the 6 lymphokine activities studied there are 3 different times at which maximal or near-maximal levels are reached: IL 2, 12-24 h; IL 3 and CSF, 24-48 h; and (Ia+)-inducing activity, MAF and interferon, 48 h or later. Only IL 2 activity disappears during the 8-day culture cycle. The time-course data and the differential production of activities by the 3 types of lymphocyte clones suggest that at least 4 terminal effector lymphokine molecules account for the 10 biologic activities tested.