Preservation of red blood cells with purines and nucleosides. II. Uptake and utilization of purines and nucleosides by stored red blood cells
Strauss, D.; de Verdier, C.H.
Folia Haematologica 107(3): 417-433
1980
ISSN/ISBN: 0323-4347 PMID: 6159280 Document Number: 167427
The uptake of adenine, guanine, guanosine and inosine by stored blood with a concentration of 100 .mu.M in plasma. Three ways of utilization of purines by stored red cells were discussed: the synthesis of nucleotide monophosphates, the formation of nucleosides, and the deamination. The relative proportions of these utilization pathways changed during storage. The most effective concentrations of adenine and guanosine in stored blood appear to be 0.25 and 0.5 mM, respectively. Full utilization of nucleosides requires the addition of Pi.