Investigations on the deformability of human red blood cells stored in different preservative solutions: influence of changed medium composition
Wegner, G.; Kucera, W.; Lerche, D.
Biomedica Biochimica Acta 46(7): 605-609
1987
ISSN/ISBN: 0232-766X PMID: 3426572 Document Number: 291765
In continuation of a former paper (W. Kucera, G. Wegner, D. Lerche: Biomed. Biochim. Acta 44, 1459 to 1467, 1985) the deformability changes of human red blood cells (RBC) during 35 days of storage in different resuspension media of changed composition were characterized by a filtration procedure. The filterability of RBC stored in CDS-AG medium (high sucrose content) decreased drastically (increase of the filterability index to more than the 6-fold). This deformability loss is caused by an increase of the internal viscosity (at least 4-fold) due to shrinkage of red blood cells by more than 20% as an effect of low ionic strength. In contrast, the filterability loss of RBC stored in SAGM medium was slight. The cell volume showed a slight increase with unchanged mean cellular hemoglobin concentration. By changing the composition of CDS-AG medium by reduction of the sucrose content from 234 mM to 30 mM and addition of 150 mM NaCl (SAGS medium) the drastic deformability loss was avoided. From the viewpoint of deformability this SAGS medium is comparable to the internationally accepted SAGM resuspension medium.