Comparison of hyaluronan binding assay scores of spermatozoa using swim-up techniques and density gradient centrifugation
Tachawiwat, K.; Getpook, C.; Geater, A.
Journal of the Medical Association of Thailand 98(Suppl 2): S84-S91
2015
ISSN/ISBN: 0125-2208 PMID: 26211109 Document Number: 16355
To compare the hyaluronan binding assay (HBA) scores of sperm preparation using two different methods, the swim-up technique and density gradient centrifugation (DGC). This experimental study used semen specimens from 54 volunteer subjects with normal semen analysis according to the 2010 World Health Organization criteria. Each semen specimen was split into two portions: one was prepared using the swim-up method and the other the DGC method. The prepared sperm were counted in the sperm-HBA slide to determine bound and unbound motile sperm. The HBA scores between the two methods were compared using matched analysis. The HBA scores by either preparation method were >80%. There was no statistically significant difference in HBA scores between the swim-up preparation [median 97%, interquartile range (IQR) 94, 98] and density gradient centrifugation [median 96%, IQR 95, 98] (p = 0.96). Ten ofthe 54 specimens received the same HBA scores following the two methods and none differed by more than +/- 7%. Both preparation methods gave high HBA scores with no apparent difference in the proportions between methods.