Density-gradient centrifugation in lithium metatungstate and tris- (hydroxymethyl) aminomethane phosphotungstate
Voronina, A.S.; Bogatyreva, S.A.
Molekuliarnaia Biologiia 14(1): 190-199
1980
ISSN/ISBN: 0026-8984 PMID: 6164914 Document Number: 162683
New substances--lithium metatungstate (MTL) and tris-(hydroxymethyl)aminomethane phosphotungstate (PTT)--have been presented for density-gradient preparation. The buoyant densities of protein, RNA, DNA and some nucleoproteins were determined in solutions of these salts. Nucleic acids have been smaller buoyant density (1.1 g/cm3) than the proteins in contrast to CsCl-gradients. The protein in PTT solution have buoyant density 1.5 g/cm3 and in MTL solution 2.0-2,3 g/cm3. It was shown that MTL gradients allow to reach better resolution in nucleoprotein analysis than CsCl gradients.