Buoyant density of some togaviruses in sucrose density gradient and capacity of their haemagglutinin fractions to interact with antibody
Kokorev, V.S.; Podoplekin, V.D.; Pereverzev, V.D.; Fedotova, T.T.
Acta Virologica 20(5): 353-360
1976
ISSN/ISBN: 0001-723X PMID: 11662 Document Number: 102372
The buoyant densities of Western equine encephalomyelitis virus (an Alphavirus) and tick-borne encephalitis (TBE) virus (a Flavivirus) antigens prepared by different methods were studied. Sucrose density centrifugation revealed a heterogeneity in the density of the virions. The sedimentation pattern and height of peaks of the hemagglutinating activities and infectivity, other conditions being equal, depended both on the virus species and properties of its strains and on the mode of preparation and treatment of the virus-containing material. Different hemagglutinating antigen fractions differed in their capacity to interact with specific antibody. The kinetics of a serological reaction and its result may depend on the functional activity of the antigen preparation and, in particular, on the proportion in the reaction mixture of virus particles with a dissimilar antigenic structure.