A method of fractionating bone marrow cells in a density gradient using a mixture of Ficoll and Verografin
Sibirnaia, N.A.; Sukhomlinov, B.F.; Khmil', M.V.
Laboratornoe Delo 4: 24-25
1991
ISSN/ISBN: 0023-6748 PMID: 1715424 Document Number: 374182
The authors suggest a method for hemopoietic tissue cell fractionation into populations enriched for various hemopoietic elements. Centrifugation in a three-layer density gradient of mixtures of ficoll and verografin (d1 = 1.03 g/cm3, d2 = 1.09 g/cm3, d3 = 1.1 g/cm3) resulted in three fractions. The first contained lymphoid cells, the second erythroid cells (89.1 +/- 7.3%), and the third consisted of white blood cells (78.5 +/- 6.3%).
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