Improved analysis of hemagglutination assays for quantitation of lectin activity
Köhle, H.; Kauss, H.
Analytical Biochemistry 103(2): 227-229
1980
ISSN/ISBN: 0003-2697 PMID: 7386857 Document Number: 163335
A method to quantitate lectin activity based on hemagglutination assay in microtiter plates was described. In addition to the normal method of visual titer evaluation an electronic particle counter was used for counting nonagglutinated cells in the microtiter wells; this allowed a rapid, quantitative determination of the amount of lectin required to agglutinate 50% of the countable single cells. Counting results should be related to a standard curve of concanavalin A to improve the reproducibility of the assay.