Effects of ACTH on the synthesis and metabolism of prostaglandin in rat kidney
Gecse, A.; Ottlecz, A.; Faragó, M.; Forster, T.; Telegdy, G.
Acta Physiologica Academiae Scientiarum Hungaricae 55(2): 107-112
1980
ISSN/ISBN: 0001-6756 PMID: 6254326 Document Number: 162377
Prostaglandin (PG) synthetase activity of rat kidney medulla microsomal fraction was determined in vitro using 1-14C-arachidonic acid as substrate. Natural ACTH resulted in a dose-dependent suppression of PGE2 formation in vitro. The biosynthesis of PGF2.alpha. was enhanced in the presence of ACTH. ACTH4-10 (1-Phe7 or d-Phe7) resulted in decreased PGE2 synthesis. The ratio of PGF2.alpha./PGE2 increased in proportion to the concentration of natural ACTH. The increase in the ratio of PG was more pronounced when ACTH4-10 fragments were applied. Natural ACTH inhibited the PG dehydrogenase activity of kidney cytosol fraction in vitro in a dose-dependent manner. PG inactivation was suppressed only by high doses of ACTH4-10 (d-Phe7). The natural ACTH and ACTH4-10 fragments might have a physiological role in the regulation of the PG system of a non-steroidogenic tissue.