Effect of mercury, lead and acetylphenylhydrazine (APHZ) on erythrocyte and bone marrow glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49) . an electrophoretic study
Dabrowski, Z.; Miszta, H.; Marszatek, K.
Folia Haematologica 107(1): 81-88
1980
ISSN/ISBN: 0323-4347 PMID: 6157628 Document Number: 161321
Male and female rats of Wistar strain have been injected with 0.2% HgCl2, 203HgCl2, PbNO3 and 2% APHZ, in the volume of 0.5 ml saline for 4 days. Subbands of G-6-PD enzyme from erythrocytes and bone marrow cells, were separated by disc polyacrylamide electrophoresis. Four subbands of the enzyme resulted electrophoretic separation. In erythrocytes increase of activity of fraction I. and decrease of fraction II were observed, while in bone marrow opposite reaction was visible. In both tissue following administration of Hg and Pb, fractions III and IV disappeared. In the place of disappeared bands increased radioactivity of 203Hg was detected.