Differentiation of Treponema hyodysenteriae from T innocens by enteropathogenicity testing in the CF1 mouse
Joens, L.A.; Glock, R.D.; Kinyon, J.M.
Veterinary Record 107(23): 527-529
1980
ISSN/ISBN: 0042-4900 PMID: 7467101 Document Number: 157828
Fourteen isolates of Treponema hyodysenteriae and 11 isolates of T. innocens from eight different countries were evaluated in the CF1 strain female mouse for virulence. Mice were fasted for 24 hours and inoculated intragastrically with 1 ml of culture for two consecutive days. Mice were killed and examined post mortem at 12 to 15 days after inoculation. Caecitis was detected in mice from each of the groups receiving T. hyodysenteriae (67%) but not in mice receiving T. innocens or sterile broth. Lesions consisted of hyperaemia, mucosal oedema, catarrhal inflammation and occasional haemorrhage. These studies suggest that the CF1 mouse may provide an inexpensive means of differentiating T. hyodysenteriae from T. innocens in vivo.