Relative activities of heparin fractions obtained by gel chromatography
Graham, D.T.; Pomeroy, A.R.
Thrombosis and Haemostasis 42(5): 1598-1603
1980
ISSN/ISBN: 0340-6245 PMID: 7368160 Document Number: 155518
To investigate the reported heterogeneity of commercial heparin injections, lung and mucous preparations were fractionated by gel chromatography according to molecular size. Eluent pools were characterized by measuring electrophoretic mobility, anticoagulant activity -Xa assay, lipolytic activity and the protamine neutralization value. The only biological activity to show a marked relationship with the molecular size of the heparin fraction was the anti-Xa activation activity. This effect was more pronounced with the mucous heparins than with the lung heparin preparation studied.