Separation of two fractions from rabbit anti-BPTi antibody by affinity chromatography
Kurowska, E.; Szewczuk, A.
Archivum Immunologiae et Therapiae Experimentalis 36(1): 7-13
1988
ISSN/ISBN: 0004-069X PMID: 2466451 Document Number: 325793
Non-competitive enzyme-linked immunosorbent assay (ELISA) for rabbit anti-BPTI antibody is described. The antibody was purified by affinity chromatography on Sepharose-BPTI column and then separated into two antibody fractions on Sepharose-trypsin (BPTI) column. The fraction I antibody, not retarded on the second biosorbent column, efficiently inactivated BPTI, but the fraction II antibody, eluted from the column with glycine buffer, pH 2.2 was firmly bound to active BPTI. This proves that BPTI has at least two epitopes.