Two-dimensional gel electrophoresis can be used to examine chromatographic effluent fractions from displacement column chromatography
Torres, A.R.; Krueger, G.G.; Peterson, E.A.
Clinical Chemistry 28(4 Part 2): 998-999
1982
ISSN/ISBN: 0009-9147 PMID: 6176365 Document Number: 187809
We show how two-dimensional gel electrophoresis can be used to monitor protein components in effluent fractions from a displacement column. A minor protein in human serum, of interest in studies on psoriasis and highly enriched by using carboxymethyldextrans as displacers on DEAE-Sephacel, was easily detected in the effluent fractions with the two-dimensional gel method because its concentration was sufficiently high and there was no interference by the carboxymethyldextrans or salt.