Inhibition of erythropoiesis by plasma component (s) from sheep, goats, and rabbits
Neal, W.A.; Lewis, J.P.; Welch, E.T.; Lutcher, C.L.; Moores, R.R.; Wright, C.S.
American Journal of Veterinary Research 40(4): 493-497
1979
ISSN/ISBN: 0002-9645 PMID: 517822 Document Number: 151245
Plasma from hypertransfused and normal sheep and experimentally induced anemic and normal goats was fractionated by ultrafiltration. Fractions obtained were assayed for erythropoeisis stimulatory factor (ESF) or erythropoiesis inhibitory factor (EIF) activity (or both) in the posthypoxic polycythemic mouse assay. The most potent sheep plasma-inhibitor fraction was found in the retentate on a membrane with a cutoff at MW 500. The yield of the most potent EIF fraction from hypertransfused sheep was higher than was the yield from normal sheep. All detectable ESF activity from the plasma of anemic goats was found in the retentate on a membrane with a cutoff at MW 50,000. The most potent EIF fraction from anemic goats passed into the ultrafiltrate, but the comparable EIF from normal goats remained in the retentate on a membrane with a cutoff at MW 500. The yield of the most potent EIF fraction was higher from anemic goats than was the yield from normal goats. During TLC, EIF extracted from goat plasma fractions had the same mobility as did prostaglandin F2.alpha. Cohn rabbit plasma fraction IV-4 had an inhibitory factor and a benzene extract of the fraction contained a component that had the same mobility as did prostaglandin F2.alpha. Cohn rabbit plasma fraction V contained a component that potentiated an erythropoietin-generating factor.