The influence of cyclophosphamide on immunity in mice infected with Trichinella spiralis
Machnicka, B.; Golińska, Z.; Bany, J.
Wiadomosci Parazytologiczne 25(5): 505-517
1979
ISSN/ISBN: 0043-5163 PMID: 543122 Document Number: 149474
Mice infected with 200 Trichinella spiralis larvae were injected intraperitoneally with 2 or 10 mg of cyclophosphamide/kg body-weight/day from the 12th hour of infection. Worms were expelled from the small intestine of control mice (not given cyclophosphamide) between the 10th and 14th days of infection; in treated mice, worms were still present after 20 days. Numbers of muscle larvae present 35 and 49 days after infection were, respectively, 20 930 and 24 900 in control mice, 23 100 and 27 780 in those given 2 mg/kg/day and 16 900 and 35 980 in those given 10 mg/kg/day. Serum IgM levels were highest (from the 7th day of infection) in mice given 2 mg/kg/day. From 14 to 42 days after infection the serum IgM level in mice given 10 mg/kg/day was lower than in controls. Up to the 42nd day of infection the control mice had the highest levels of IgG. The 10 mg/kg/day dose of cyclophosphamide had a marked suppressive effect on IgA levels. Haemagglutination titres were positive in the control mice on the 10th day, but remained negative in the other 2 groups until day 35.