Comparative Studies on Nucleic Acid Based Biosensors for Identification of Filarial Nematode
Jaratsing, P.; Viseshakul, N.; Areekit, S.; Chansiri, K.
Journal of the Medical Association of Thailand 99(Suppl 8): S216-S221
2016
ISSN/ISBN: 0125-2208 PMID: 29906048 Document Number: 14643
The microfilarial nematodes, found mainly in blood circulation, form a special group of human and veterinary To compare the sensitivity, specificity and accuracy of NABs with standard detection methods. In this study, the microfilariae of Dirofilaria immitis was used as the representative model for filarial nematode. The PCR and LAMP primers were designed and synthesized according to the specific nucleotide regions of a small subunit gene of the parasite as well as the DNA probes. The fifty unknown blood samples were sent as a gift from Prasu Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University. The samples were tested by using PCR, PCRLFD, PCR-AuNP, LAMP, LAMP-LFD, LAMP-AuNP, LAMP-turbidity and film blood smear. The efficacy of NABs was compared to a standard film blood smear in terms of sensitivity, specificity and accuracy. Upon detection of fifty unknown blood samples, LAMP-LFD assay presented 100% of sensitivity, specificity and accuracy. The data revealed that sensitivity, specificity and accuracy of NABs varied from 66.67-100.00% when compared to a film blood smear stained with Giemsa dye. The data clearly indicated that LAMP-LFD was a preferred choice for the use as a point-of-care NABs. NABs were highly sensitive performing as a selective diagnostic tool that could be applied particularly as the