Preservation of buffalo semen at variable room temperature in some modified diluents
Vidyachand, E.; Sengupta, B.P.
Indian Veterinary Journal 56(4): 289-293
1979
ISSN/ISBN: 0019-6479 PMID: 489108 Document Number: 141843
Semen from 6 Murrah buffalo bulls was diluted in the following diluents: (1) coconut milk extender (CME); (2) CME + cysteine hydrochloride; (3) CME + glucose; (4) CME + glucose + cysteine hydrochloride; (5) modified Cornell University Extender (CUE) + cysteine hydrochloride; (6) modified IVT diluent + cysteine hydrochloride. After 48 h of storage at room temp., sperm motility was significantly higher for diluent 2 than for all other diluents (56.9 v 53.0-55.5%), but after 96 and 144 h there were no significant differences. In a 2nd experiment, diluents 2, 5 and 6 were used with the addition of either 150 units catalase/ml (a) or 1 mg sodium pyruvate/ml (b). After all storage intervals, differences in sperm motility were highly significant between diluents. For diluents 2a, 2b, 5a, 5b, 6a and 6b resp., sperm motility after 144 h of storage was 35.7, 40.4, 45.4, 50.3, 44.2 and 47.7%.