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Isolation and purification of moniliformin

Steyn, M.; Thiel, P.G.; van Schalkwyk, G.C.

Journal - Association of Official Analytical Chemists 61(3): 578-580

1978

ISSN/ISBN: 0004-5756
PMID: 649549
Document Number: 131929
A bulk purification procedure is described for moniliformin, a mycotoxin produced by Fusarium moniliforme. The method involves methanol extraction of suitably molded maize, aqueous extraction of the methanol-f ree residue, ion exchange chromatography with a NaCl concentration gradient, desalination, and crystallization. A pKa value of 1.70 as well as molar absorptivities of 19100 (λ(H2O)max 229 nm), 5600 (λ(H2O)max 260 nm), and 4700 (λ(MeOH)max 260 nm) L/mole/cm are reported for moniliformin.

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