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Isolation and purification of virus damaging sunflower

Zakusilo, A.O.; Didenko, L.F.; Kniazieva, N.A.; Boĭko, A.L.

Ukrainskii Biokhimicheskii Zhurnal 66(5): 87-92

1994

ISSN/ISBN: 0201-8470
PMID: 7747352
Document Number: 435469
A procedure has beer, developed for purifying intact virus's isolate particles evoking yellow spot mosaic disease in sunflower. Purification of pathogen in 0,1 M sodium phosphate buffer, pH 8.0 containing 0.05 M Na-3SO-3 and 0.2% 2-mercaptoethanol is used. After first clarification extract was exposed to two cycles of high-speed centrifugation and fractionated in linear 10-40% (wt vol-1) sucrose density gradient. Virus was recovered from appropriate fractions after dialysis against 0.01 M Na-2SO-3.

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