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Isolation and purification of Ca2+,Mg2+-ATPase from plasma membranes of the myometrium

Slinchenko, N.N.; Liubakovskaia, L.A.; Kurskiĭ, M.D.; Sopel', L.V.

Ukrainskii Biokhimicheskii Zhurnal 62(3): 60-65

1990

ISSN/ISBN: 0201-8470
PMID: 2144381
Document Number: 357158
The preparation of the purified Ca2+, Mg2(+)-ATPase has been isolated from triton X-100 solubilizate of plasma membranes of the pig myometrium using the method of affinity chromatography on calmodulin-Sepharose 4B. The specific activity of the enzyme shows its 52-fold purification. The enzymic preparation practically has no Mg2(+)-ATPase activity. By the data of DS-Na-electrophoresis in PAAG the Ca2+, Mg2+ ATPase preparation consists of two polypeptides with Mm 130 and 205 kDa. Autoradiography shows their Ca2(+)-dependent phosphorylation. The purified enzyme is highly sensitive to the inhibitory effect of orthovanadate.

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Kondratiuk, T.P.; Prishchepa, L.A.; Kurskiĭ, M.D.; Osipenko, A.A. 1988: Calmodulin-dependent regulation of Ca,Mg-ATPase activity in plasma membranes of the swine myometrium Biokhimiia 53(5): 793-798
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Veklich, T.O.; Kosterin, S.O. 2005: Comparative study of properties of Na+, K+-ATPase and Mg2+-ATPase of the myometrium plasma membrane Ukrains'kyi Biokhimichnyi Zhurnal 77(2): 66-75
Dreval', V.I. 1992: Postradiation changes in Ca2+-ATPase and Mg2+-ATPase in thymocyte plasma membranes Ukrainskii Biokhimicheskii Zhurnal 64(5): 103-106
Prishchepa, L.A.; Kondratiuk, T.P.; Kurskiĭ, M.D. 1989: Ca2+ (calmodulin)-dependent phosphorylation of plasma membranes of pig myometrium Ukrainskii Biokhimicheskii Zhurnal 61(1): 85-89
Veklich, T.O.; Shkrabak, O.A.; Mazur, I.I.; Rodik, R.V.; Boĭko, V.I.; Kal'chenko, V.I.; Kosterin, S.O. 2013: Kinetic properties of calixarene C-90 action on the myometrial plasma membrane Ca2+,Mg2+-Atpase activity and on the Ca2+ concentration in unexcited cells of the myometrium Ukrains'kyi Biokhimichnyi Zhurnal 85(4): 20-29
Kurskiĭ, M.D.; Kondratiuk, T.P.; Babich, L.G.; Bychenok, S.F. 1987: CAMP-dependent regulation of the active transport of Ca2+ in plasma membranes of the swine myometrium Biokhimiia 52(10): 1618-1623
Shlykov, S.G.; Slinchenko, N.N.; Kurskiĭ, M.D. 1987: Properties of adenylate cyclase from plasma membranes of the rabbit myometrium in different functional states Ukrainskii Biokhimicheskii Zhurnal 59(1): 82-86
Danylovych, H.V.; Danylovych, I.V. 2007: Effect of nitric oxides and hydrogen peroxide on Ca2+, Mg(2+)-ATPase and Mg(2+)-ATPase activity in myometrium sarcolemma Ukrains'kyi Biokhimichnyi Zhurnal 79(2): 30-37
Ryskulova, S.T.; Ivashchenko, A.T. 1980: Effect of adrenaline on the ATPase activity of rat liver plasma membranes Voprosy Meditsinskoi Khimii 26(3): 308-312
Dreval', V.I. 1993: Effect of ionizing radiation on ATPase activity of thymocyte plasma membranes Ukrainskii Biokhimicheskii Zhurnal 65(2): 99-102
Tsvilikhovskiĭ, N.I.; Usatiuk, P.V.; Mel'nichuk, D.A. 1988: Isolation, purification and characteristics of brush border and basolateral membranes from cattle intestinal epithelium cells Ukrainskii Biokhimicheskii Zhurnal 60(6): 91-94
Makogonenko, E.M.; Grinenko, T.V.; Kudinov, S.A. 1974: Effect of SH-reagents on the Mg2+-dependent Ca2+-activated ATPase activity of synaptosome plasma membranes Ukrains'kyi Biokhimichnyi Zhurnal 46(5): 553-560
Veklich, T.O.; Kosterin, S.O.; Rodik, R.V.; Cherenok, S.O.; Boĭko, V.I.; Kal'chenko, V.I. 2006: Effect of calixarene-phosphonic acid on Na+, K+-ATPase activity in plasma membranes of the smooth-muscle cells Ukrains'kyi Biokhimichnyi Zhurnal 78(1): 70-86
Luppa, D.; Müller, F. 1982: Increase of (Na+ + K+)-activated ATPase activity of basolateral plasma membranes from intestinal mucosa of diabetic rats Acta Biologica et Medica Germanica 41(10): 891-898
Pleten', A.P.; Mazo, V.K.; Nikulicheva, S.I.; Shaternikov, V.A. 1978: Isolation and purification of angiotensinogen from swine and sheep plasma Voprosy Meditsinskoi Khimii 24(6): 798-801
Sharma, A.; Ray, T.K. 1982: Isolation of lactating goat mammary plasma membranes Indian Journal of Experimental Biology 20(3): 257-259
Fendler, K.; van der Hijden, H.; Nagel, G.; de Pont, J.J.; Bamberg, E. 1988: Pump currents generated by renal Na+K+-ATPase and gastric H+K+-ATPase on black lipid membranes Progress in Clinical and Biological Research 268a: 501-510
Pokudin, N.I.; Orlov, S.N.; Postnov, I.V. 1986: The Ca pump and Ca ATPase in the erythrocytes of patients with hypertension: disorders detected in cytoskeletal membranes and in solubilized Ca ATPase Kardiologiia 26(3): 94-99