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Influence of protein bound with single-stranded DNA on the synthesis of RNA and poly (A) . III. Protein product of F1 gene 5

Polonskiĭ, I.S.; Naĭdenova, N.M.; Kozlov, I.I.; Zograf, I.N.; Debabov, V.G.

Molekuliarnaia Biologiia 11(3): 656-660

1977

ISSN/ISBN: 0026-8984
PMID: 379607
Document Number: 123833
Influence of the protein product of F1 phage gene 5 (protein 5) on the synthesis RNA and poly(A) in vitro was studied. It has been shown, that protein 5 has no effect on the transcription of the native DNA by E. coli RNA-polymerase, but completely prevents RNA and poly(A) synthesis on the denatured or single-stranded DNA at the protein/DNA ratio 10:1. Protein 5 inhibits poly(A) synthesis with oligo(dT)9 and oligo(dT)12 as a template, preventing binding of enzyme to the oligonucleotide. After the initiation of the poly(A) synthesis the inhibition becomes considerably wearer. The biological function of the inhibition of the transcription by "unwinding" proteins is discussed.

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Zlochevskiĭ, M.L.; Kozlov, I.I.; Debabov, V.G. 1978: Complex of single-stranded phage DNA and protein--a product of bacteriophage fl gene 3. Distribution of gene 3 protein in the DNA molecule Molekuliarnaia Biologiia 12(3): 505-514
Koterov, A.N. 1997: Effect of zinc-metallothionein on DNA replicative synthesis and single-stranded DNA-binding protein (SSB-protein) content in chromatin of mice bone marrow cells Ukrainskii Biokhimicheskii Zhurnal 69(1): 84-87
Veĭko, N.N.; Gromova, E.S.; Shabarova, Z.A. 1979: Interaction of gene 5 protein of phage f1 with single and double-stranded DNA and polynucleotides Molekuliarnaia Biologiia 13(5): 1136-1146
Williams, R.T.; Carbonaro-Hall, D.A.; Hall, F.L. 1992: Co-purification of p34cdc2/p58cyclin A proline-directed protein kinase and the retinoblastoma tumor susceptibility gene product: interaction of an oncogenic serine/threonine protein kinase with a tumor-suppressor protein Oncogene 7(3): 423-432
Timofeev, V.P.; Arutiunian, A.I.; Petrov, A.I. 1990: Segmental flexibility of single-, double-, and triple-stranded polyribonucleotides from the data of spin label method. Formation of the triple-stranded poly(A.A.U) polynucleotide helix Molekuliarnaia Biologiia 24(1): 140-155
Hewat, E.A.; Ruigrok, R.W.; DiCapua, E. 1991: Activation of recA protein: the pitch of the helical complex with single-stranded DNA EMBO Journal 10(9): 2695-2698
Chernyĭ, A.A.; Lysov, I.P.; Il'icheva, I.A.; Zibrov, A.S.; Shchelkina, A.K.; Borisova, O.F.; Mamaeva, O.K.; Florent'ev, V.L. 1990: Four-stranded DNA. Conformational analysis of regular spirals of poly(dT).poly(dA).poly(dA).poly(dT) with different base binding variations Molekuliarnaia Biologiia 24(5): 1399-1410
Permogorov, V.I.; Tiaglov, B.V.; Minaev, V.E. 1980: Study of fusion of bacteriophage f2 double-stranded RNA, poly(A).poly(U), and poly(G).poly(C) in the presence of tetraethylammonium bromide Molekuliarnaia Biologiia 14(5): 1013-1018
Kishore, U.; Chauhan, M.; Choudhury, N.R.; Khatri, G.S. 1994: Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter Indian Journal of Biochemistry and Biophysics 31(4): 249-253
Chambers, J.A.; Hinkelammert, K.; Russo, V.E. 1985: Light-regulated protein and poly(A)+ mRNA synthesis in Neurospora crassa EMBO Journal 4(13B): 3649-3653
Katchalski-Katzir, E. 1996: Synthesis, physicochemical and biological properties of poly-alpha-amino acids--the simplest of protein models Acta Biochimica Polonica 43(1): 217-226
Kuzela, S.; Kolarov, J.; Krempaský, V. 1973: Electrophoretic properties of the product of protein synthesis in mitochondria of rat liver and Zajdela hepatoma Neoplasma 20(6): 623-630
Ratovitskiĭ, E.A.; Rudenko, A.B.; Vasinova, N.A.; Kidgotko, O.V.; Nisman, B.K. 1990: Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 20-24
Koyama, T. 1972: Protein synthesis in the lens and its disturbance. 3. Influence of the basic condition on the accumulation of 14 C-L-leucine by rabbit lenses and the rate of reduction of 14 C-L-leucine incorporated into lens protein Nippon Ganka Gakkai Zasshi 76(8): 709-717
Hattori, S.; Maekawa, M.; Nakamura, S. 1992: Identification of neurofibromatosis type i gene product as an insoluble GTPase-activating protein toward ras p21 Oncogene 7(3): 481-485
Robbins, K.C.; Leal, F.; Pierce, J.H.; Aaronson, S.A. 1985: The v-sis/PDGF-2 transforming gene product localizes to cell membranes but is not a secretory protein EMBO Journal 4(7): 1783-1792
Chou, J.; Lemaux, P.G.; Casadaban, M.J.; Cohen, S.N. 1979: Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product Nature 282(5741): 801-806
Iurkevich, I.V. 1980: Influence of the pituitary gland on levels of total protein-bound and unbound 11-hydroxycorticosteroids during the course of the compression syndrome Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia 3: 55-57
Sorio, C.; Mendrola, J.; Lou, Z.; LaForgia, S.; Croce, C.M.; Huebner, K. 1995: Characterization of the receptor protein tyrosine phosphatase gene product PTP gamma: binding and activation by triphosphorylated nucleosides Cancer Research 55(21): 4855-4864
Yavuzer, U.; Keenan, E.; Lowings, P.; Vachtenheim, J.; Currie, G.; Goding, C.R. 1995: The Microphthalmia gene product interacts with the retinoblastoma protein in vitro and is a target for deregulation of melanocyte-specific transcription Oncogene 10(1): 123-134