Serologic and immunogenic properties of staphylococcal protein a
Tolovskaia, K.R.; Akatov, A.K.; Prokhorov, V.I.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 10: 51-54
1977
ISSN/ISBN: 0372-9311 PMID: 411285 Document Number: 119360
Protein antigen A was isolated from the microbial cells of Cowan I strain of Staphylococcus aureus by hot extraction method. Ion exchange chromatography on DEAE-cellulose-32 and gel-filtration on sephadex F-100 were used for purification of protein A. Microprecipitation in agar test against homologous immune rabbit serum and normal human serum showed purified protein A to be identical by serological specificity to the standard preparation obtained from Prof. Oeding's laboratory. In order to assess the immunogenic properties of protein A by various doses of the preparation (from 2 to 1000 microgram) mixed with A1 (OH)3 albino mice were immunized and then infected with the microbial culture of the homologous strain. As revealed, protein A not only failed to protect the animals from death, but even aggravated the course of infection.