Immunogenic properties of soluble cytosol fractions on Meth A sarcoma cells
Dubois, G.C.; Appella, E.; Law, L.W.; Deleo, A.B.; Old, L.J.
Cancer Research 40(11): 4204-4208
1980
ISSN/ISBN: 0008-5472 PMID: 7471061 Document Number: 166363
Tumor-associated transplantation antigen (TATA) was present in fractions derived from the cytosol of the Meth A cell. Meth A ascites cells were disrupted, nuclei and membranes were removed by low- and high-speed centrifugation, and the soluble protein was fractionated by ammonium sulfate precipitation and gel filtration chromatography. The TATA of the soluble cytosol fractions appears to be identical with the TATA solubilized from plasma membranes. The TATA of the cytosol fractions was associated with proteins of an apparent MW of .apprx. 60,000, specific for the Meth A tumor, and as immunogenic as the membrane-derived TATA. The most enriched TATA cytosol fraction shows inhibition of an antiserum capable of detecting a tumor-specific surface antigen of Meth A. Meth A TATA apparently is not an integral membrane protein and may be related to the tumor-specific surface antigen detected serologically.