Incorporation of 14C-amino acids into rat hypothalamus in vitro
Kordić, D.; Kniewald, Z.
Endocrinologia Experimentalis 11(1): 3-10
1977
ISSN/ISBN: 0013-7200 PMID: 322995 Document Number: 117717
Biosynthesis of peptides in vitro in male rat hypothalamic fragments wasinvestigated by incubating the tissue with 14C-labelled leucine, proline and glycine. Depending on the amino acid used, 4 or 6 labelled peptides were extracted from the tissue fragments with organic solvent mixture. The incorporation of the label into peptides was time dependent. LH-RH and oxytocin thus synthesized were identified by thin layer chromatography and high voltage electrophoresis using cold LH-RH and oxytocin as standards.