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The incorporation of labelled amino acids into the subcellular fractions of the rabbit brain

Ogrodnik, W.

Annales Universitatis Mariae Curie-Sklodowska. Sectio D Medicina 35: 207-216

1980

ISSN/ISBN: 0066-2240
PMID: 6185096
Document Number: 163878
Radioactive amino acids were injected into the 4th ventricule of adult rabbits. After 3, 6 and 13 h the animals were killed and tissue subcellular fractions were prepared from their brains. Nucleic acids were extracted and quantitatively determined from nucleic, myelin, mitochondrial, microsomal and cytoplasmic fractions. The radioactivity was determined in the protein and nucleic acid fractions. The incorporation of radioactive amino acids increased in relation to time. In the analyzed subcellular fractions a very rapid incorporation of Glu and Leu into cytoplasmic proteins was observed. The chromatographic analysis of the nucleic acids showed that radioactivity in the nucleic acid fractions depended on a radioactive protein contamination. Radioactive aminoacyl-tRNA was not found in the nucleic acid fractions extracted from different subcellular fractions.

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