A gas chromatography-mass fragmentographic method for the assay of ajmaline and its monochloroacetyl ester
Clemans, S.D.; Davison, C.; Koss, R.F.; Dorrbecker, B.; O'Melia, P.E.; Ross, R.W.; Edelson, J.
Arzneimittel-Forschung 27(6): 1128-1130
1977
ISSN/ISBN: 0004-4172 PMID: 578424 Document Number: 116121
A gas chromatograph-mass fragmentography method for simultaneous assay of 17-monochloroacetyl-ajmaline (MCAA) and its hydrolysis product, ajmaline, is described. Recovery of both compounds from whole blood averaged 71%. About 5% of MCAA was hydrolyzed during the assay procedure. The method was accurate and precise to within a few percent. It was suitable for assays of blood levels in the dog after an i.v. dose of 2 mg/kg or an oral dose of 5 mg/kg.