A simple method for determination of flurbiprofen in human plasma by gas chromatography-mass spectrometry
Satomoto, M.; Adachi, Y.; Higuchi, H.; Watanabe, K.; Satoh, T.
Masui. Japanese Journal of Anesthesiology 51(4): 431-434
2002
ISSN/ISBN: 0021-4892 PMID: 11995356 Document Number: 546412
A method for gas chromatograph-mass spectrometric assay has been developed for the quantitation of flurbiprofen in human plasma. Extraction or condensing procedure was not required and the method reduced time involved in sample preparation. The analytes were separated on the fused-silica capillary column. The operating conditions were: injector, 250degreeC; detector, 300degreeC; and column, 50-280degreeC. The total gas flow-rate of helium (carrier) was 50 mlcntdotmin-1 and the pressure of column inlet was 100-200 kPa. The retention time was 18.1 min and the limit of quantitation was 0.5 mugcntdotml-1. This method provides an easy and simple method for the detection of flurbiprofen.