Immunoglobulin response to bluetongue virus soluble antigen in subcutaneous chambers
Hajer, I.; Jochim, M.M.; Lauerman, L.H.
American Journal of Veterinary Research 38(6): 815-818
1977
ISSN/ISBN: 0002-9645 PMID: 195497 Document Number: 115515
Group-specific antibodies were produced by inoculation of bluetongue virus soluble antigen into polyethylene chambers implanted subcutaneously in 8 rabbits and 2 sheep. For comparison, 5 rabbits and 1 sheep were inoculated intramuscularly with the soluble antigen in Freund's complete adjuvant. Antibodies present in the serum and chamber fluids were detected by the agar gel precipitin or serum-neutralization tests, qualitatively examined by immunoelectrophoresis and immunofluorescence, and quantitated by electroimmunodiffusion.