Detection of immunoglobulin G to a Sindbis-related virus by a membrane antigen enzyme immunoassay
Scalia, G.; Garufi, M.; Condorelli, F.; Stivala, A.; Costanzo, M.C.; Tiralongo, M.; Adragna, A.D.; Marino, A.; Guglielmino, S.; Tempera, G.; Castro, A.
New Microbiologica 19(1): 91-96
1996
ISSN/ISBN: 1121-7138 PMID: 8673858 Document Number: 467961
We determined the seroprevalence of a Sindbis-related virus isolated for the first time in 1975 from ticks in south-east Sicily and typed by Gresikova et al. in 1978. An indirect enzyme immunoassay based on viral membrane antigen for coating microliter strips was used for the detection of immunoglobulin G to the Sindbis-related virus. The method appeared more sensitive than a similar enzyme immunoassay based on crude lysate antigen. Comparison of the results obtained from sera tested both by membrane antigen enzyme immunoassay and microneutralization test showed 92% agreement, while the agreement between microneutralization test and crude antigen enzyme immunoassay was 76%. An overall elevated seroprevalence (63.66%) was found in a population group living in and around the area of first isolation and seroprevalence in different age groups was also studied.