Recovery of continuous cells following prolonged storage at the temperature of liquid nitrogen
Glinskikh, P.P.; Zusman, F.Ia.
Tsitologiia 18(7): 886-890
1976
ISSN/ISBN: 0041-3771 PMID: 982589 Document Number: 108908
The recovery process of HeLa continuous cells after prolonged storage at nitrogen liquid temperature (--196 degrees C) has been analysed. The karyologic analysis evidence indicates a high stock cell karyostability. A study of cell division, generation cycle parameters and DNA synthesis by autoradiography demonstrated cell adaptation to a continuous passage to be due to the increase of proliferative pool and to the intensitification of generation processes in the multiplicated population. Cell structure changes during adaptation were reversible.