Split-dose and liquid-holding recovery after X-irradiation in diploid yeast Saccharomyces cerevisiae. II. Dependence of the recovery process on cellular protein metabolism
Wienhard, I.; Kiefer, J.
International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 32(1): 33-41
1977
ISSN/ISBN: 0020-7616 PMID: 328419 Document Number: 114795
In diploid yeast, split-dose recovery (SDR) after X-irradiation was affected, if incubation between split doses was performed in the presence of the protein-synthesis inhibitor, cycloheximide. In exponentially-growing cell-cultures, early SDR was undisturbed but complete recovery was not achieved. Concomitantly the cells show a decresing ability to perform subsequent liquid-holding recovery (LHR). In stationary-phase cell-cultures, SDR was completely suppressed. The cells show, however, recovery from potentially lethal damage in the presence of cycloheximide during incubation between the dose-fractions. The experimental results suggest that in diploid yeast SDR after X-irradiation is an enzymatic process dependent on a functioning protein metabolism.