A probabilistic approach to the analysis of the frequency of diploid formation in yeast (Saccharomyces cerevisiae) mating

Kopociński, B.; Lachowicz, T.M.

Mikrobiolohichnyi Zhurnal 56(6): 3-16

1994


ISSN/ISBN: 0201-8462
PMID: 7894727
Document Number: 433617
Conditions of optimizing mating efficiency in the yeast. Saccharomyces cerevisiae were tested and measured as a number of prototrophic diploids. It was found that the process of diploid formation could be controlled by suitably adjusting cell densities in liquid mating medium and alpha:a mating type cell ratio. When the first was about 5 cntdot 10-3 per ml and the second 1:10, respectively, every alpha cell was able to find a partner of opposite mating type. Some mathematical models of the "courtship" in yeast were advanced and the conclusions are discussed in the light of recent data on mating pathway in S. cerevisiae.

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