Rec41--a new gene, participating in the control of recombination in Saccharomyces cerevisiae yeast
Chepurnaia, O.V.; Kozhina, T.N.; Peshekhonov, V.T.; Korolev, V.G.
Genetika 29(2): 246-256
1993
ISSN/ISBN: 0016-6758 PMID: 8486254 Document Number: 415069
We isolated a collection of rec- mutants. The mutant rec41 from this collection was studied in detail. The mutant demonstrated a reduced level of interplasmid recombination, did not grow at the elevated temperature (36 degrees C) and was sensitive to gamma-rays but not to ultraviolet irradiation. The rec41-1 mutation behaved as a semidominant nuclear mutation. Our study on inter- and intraplasmid recombination suggested that the rec41-1 mutation decreased the level of crossing over events significantly and the frequency of gene conversion events at a lower degree. We postulate that rec41 mutation reduces the efficiency of double-stranded break repair and this results in high sensitivity of mutant cells to methylenmethan-sulfonate and ionizing radiation. Using gamma-sensitivity of the mutant cells we isolated a rec41-1 complementing plasmid from a yeast genomic DNA library. The mapping of plasmid integration site allowed us to localize the REC41 gene on the left arm of the VII chromosome nearby LYS4 and CDC43 genes.