Prolonged storage of erythrocytes
Nowicki, P.; Kłos, M.
Acta Haematologica Polonica 7(4): 293-302
1976
ISSN/ISBN: 0001-5814 PMID: 998140 Document Number: 100968
The authors report the results of biochemical investigations of erythrocytes stored for 70 days at 4 degrees C. For regeneration of phosphate substances in the erythrocytes they were subjected to 2-hour incubation at 37 degrees C parellelly in 3 types of rejuvenating fluids (PIGP, PIGPA, PIGPAd). Regeneration was done on the 28th, 42nd, 56th and 70th days and its results were assessed on the basis of determinations of ATP, 2,3-DPG, erythrocyte osmotic resistance index, percent of their recovery and contents of free haemoglobin. These investigations were carried out immediately after incubation and after 48 hours. The best regenerative properties among these fluids had PIGPAd fluid which raised the levels of ATP and 2,3-DPG in erytrocytes up to the 70th day of storage. In the light of these results it seems that the time of erythrocyte storage can be prolonged o 70 days despite ever increasing erythrocyte loss with prolonging storage.